Journal: Biomedicines

Article Title: Mitochondrial Ribosome Dysfunction in Human Alveolar Type II Cells in Emphysema

doi: 10.3390/biomedicines10071497

Figure Lengend Snippet: Humanin expression in ATII cells. ( A ) Lung sections were obtained from nonsmokers (NS), smokers (SM), and emphysema (EM). Staining using SP-C (green) and humanin (red) antibodies and DAPI (blue) was performed by immunofluorescence using confocal fluorescence microscopy. ( B ) Relative humanin protein expression was normalized to DAPI intensity in ATII cells identified using SP-C staining. Data are presented as means ± s.e.m (N = 4–14 per group; * p < 0.05; ** p < 0.01, scale bar, 50 µm).

Article Snippet: Human lung tissue sections were stained with humanin (NB100-56876, Novus Biologicals) and SP-C antibodies.

Techniques: Expressing, Staining, Immunofluorescence, Fluorescence, Microscopy

Journal: Journal of the Endocrine Society

Article Title: Humanin Treatment Protects Against Venetoclax-Induced Bone Growth Retardation in Ex Vivo Cultured Rat Bones

doi: 10.1210/jendso/bvae009

Figure Lengend Snippet: Effects of venetoclax (Ven) and/or HNG treatment on protein expression in ex vivo cultured human growth plate tissue. A, Representative images of human growth plate tissues treated with Ven, HNG, or the combination of Ven/HNG for 48 hours and analyzed by immunohistochemistry. Alcian blue was used as counterstain. Original magnification 20×, scale bar = 100 μm. B-D, Quantification of p53, PCNA, and humanin protein expression. Individual cultures of 3 different pieces of growth plate tissue were analyzed. Data are shown as means ± SDs, n = 3/group; ** P less than .01.

Article Snippet: The primary antibodies used in the study were the following: anti-proliferating cell nuclear antigen (PCNA) antibody (1:3000; Abcam catalog No. ab18197, RRID:AB_444313), anti-Bax antibody (1:500; Abcam catalog No. ab32503, RRID:AB_725631), anti-Bcl-2 antibody (1:100; Abcam catalog No. ab196495, RRID:AB_2924862), humanin antibody (1:100; Novus catalog No. NB100-56877, RRID:AB_838379), and p53 antibody (1:200; Santa Cruz Biotechnology catalog No. sc-393031, RRID:AB_3083496).

Techniques: Expressing, Ex Vivo, Cell Culture, Immunohistochemistry

Journal: International Journal of Molecular Sciences

Article Title: Impaired Expression of Humanin during Adrenocortical Carcinoma

doi: 10.3390/ijms25021038

Figure Lengend Snippet: The tissue microarray slide (TMA) was used to analyze the expression of humanin protein in the human adrenal gland disease spectrum, focusing on various types of adrenal cancer. The localization of humanin protein was visualized through immunohistochemical staining (DAB) ( A ). The relevant types of adrenal cancer progression are indicated on the TMA map using distinct colors ( B ).

Article Snippet: The TMA slides were incubated with an anti-Humanin antibody with 1:250 dilution (#25886-1AP; Proteintech Group, Inc.; Rosemont, IL, USA) at 4 °C overnight.

Techniques: Microarray, Expressing, Immunohistochemical staining, Staining

Journal: Cell Reports Medicine

Article Title: Myeloid cells coordinately induce glioma cell-intrinsic and cell-extrinsic pathways for chemoresistance via GP130 signaling

doi: 10.1016/j.xcrm.2024.101658

Figure Lengend Snippet:

Article Snippet: Rabbit anti-Humanin , Biorbyt , Cat# orb157596.

Techniques: Plasmid Preparation, Recombinant, Transfection, Fluorescence, Staining, Reverse Transcription, Gene Expression, Liposomes, Mutagenesis, shRNA, Control, Construct, Software, Imaging, Functional Assay, Dissection, Sequencing, Real-time Polymerase Chain Reaction